ISOLATION OF OIL DEGRADING BACTERIA FROM OIL CONTAMINATED SOIL AND EXPRESSION OF OIL DEGRADING GENES IN NON OIL DEGRADING BACTERIA

Authors

  • Amit Pandey MRD LIfeSciences Pvt. Ltd, Lucknow (U.P.), 226010.

Keywords:

Bioremediation, Shake flask method, Agar well diffusion method, Spread plate method and optimization.

Abstract

The present study is carried out by isolation and characterization of oil degrading  microbes from oil contaminated sites. In this work the Bacillus megaterium , Pseudomonas fluorescenes  and  S.aureus were isolated according to Bergey’s manual and further used for oil degradation to check the production of biosurfactant. The Shake flask method, Agar well diffusion method and Spread plate method were used for oil degradation. Further the optimization was carried out for best carbon, nitrogen sources and also for pH and temperature. And in last the plasmid was isolated from production media and after restriction digestion , ligation the transformation was done to transform oil degrading genes in to non oil degrading microbes.

 

Key words: Bioremediation, Shake flask method, Agar well diffusion method, Spread plate method and optimization.

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Published

2013-11-30

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Section

Articles