FORMULATION AND EVALUATION OF BIOADHESIVE GEL INCORPORATED METRONIDAZOLE LOADED MICROSPHERES FOR PERIODONTAL THERAPY
Gingivitis and periodontitis are the two most common infectious periodontal diseases. Gingivitis occurs in almost all individuals. Bacteria that are associated with periodontal health include primary orearly colonizers such as S.sanguis, S.mitis, Gemellaspp, Atopobiumspp, Fusobacteriumnucleatum, and Capnocytophaga spp. Species belongingto the genera Veillonella, Streptococcus, and Capnocytophagaarethought to be beneficial to the host. The rationale use of metronidazole in the treatment of periodontal diseases It is also used in treatment of acute necrotizing ulcerative gingivitis and severe odontogenic infections. Excipients selected for the formulation of microspheres on the basis of their properties in which gelatin have Gelling ability, gelatin gels independently of pH and without the need for other reactive agents, Carbopol polymer is a cross-linked polyacrylate polymer that offer significant functional benefits in applications requiring flexible rheological properties and suspension control. Polysorbate containing 20 units of oxyethylene are hydrophilic nonionic surfactants, Gelatin microspheres loaded with 100 mg metronidazole was added to the drug carbopol solution and mixed well. Sodium metabisulphate (0.1 %w/w)) were then added to microsphere/drug/carbopol mixture, while stirring at 250 rpm, to obtain a homogenous mixture. Stirring was continued until a lump-free suspension was obtained. Thereafter, 0.33 ml of triethanolamine was added to produce a gel. Concentrations of drug were calculated from the standard calibration curve prepared in pH 6.8 phosphate buffers,The release study was carried out by using egg membrane. Prior the permeation experiment, membrane was clamped between the donor and the receptor compartment of the diffusion cell apparatus.
Key words- Periodontitis, Metronidazole, Bioadhesive Gel, Microspheres, Periodontal Therapy.