Analytical Method Development and Validation of Antimicrobial Agent by RP HPLC

Abstract

To determine thirteen specific pharmaceutical compounds (metformin, amoxicillin, chloroquine, theophylline, trimethoprim, caffeine, norfloxacin, ciprofloxacin, acetylsalicylic acid, doxycycline hyclate, metronidazole, albendazole, and cloxacillin) in bulk and tablet dosage form, this study set out to develop and validate an HPLC method. The chosen target compounds were completely separated by chromatographic separation using a Kromasil C18 column, gradient elution with aqueous formic acid (0.1%), methanol, and acetonitrile, a UV absorption wavelength of 250 nm, and a mobile phase flow rate of 1 mL/min over a 22-minute run time. The author has provided a quick overview of the history of chromatography, the many kinds of chromatographic methods, uses of chromatography, and the requirement of medicines in human existence. Additionally, the author has briefly explained the significance of analytical chemistry in the pharmaceutical sector. This chapter specifies the goal of the current study and primarily explains the RP-HPLC procedure, the processes in method development, and the validation parameters of the suggested technique