Evaluation of in-vitro cytotoxicity studies of Sida rhombifolia leaves extract

Abstract

Recent research into medicinal plants has led to the identification of numerous bioactive compounds with anticancer potential. Sida rhombifolia, a traditionally used medicinal herb, was evaluated in this study for its in vitro cytotoxic activity against Dalton’s Ascitic Lymphoma (DAL) cell lines using the MTT assay. Various extracts of the leaves—including ethyl acetate, aqueous, and others—were tested to determine their ability to inhibit cell viability. Among them, the ethyl acetate and aqueous extracts exhibited the most significant cytotoxic effects, indicating their potential as sources of natural anticancer compounds. These findings support further investigation into the phytochemical constituents of Sida rhombifolia and their mechanism of action in cancer therapy.

Keywords: Sida rhombifolia, MTT assay, Invitro cytotoxicity, DAL cell lines, Medicinal plants.